Spectrofluorometer

The PTI QuantaMaster series spectrofluorometer is a modular system with capabilities for measuring many luminescence phenomena. An arc lamp provides illumination for steady state measurements for observing both emission and excitation scans. A Xenon arc lamp provides pulsed illumination for measuring phosphorescence lifetimes. Additionally, several laser and LED sources are available at specific wavelengths that can be attached for collecting fluorescence lifetime measurements. The FelixGX software can be used to collect and analyze excitation and emission scans, excitation and emission ratios, timebased scans for single samples or up to 10 dyes simultaneously, lifetime measurements, and quantum yield. Furthermore, the FelixGX software can calculate a corrected spectrum to account for non-constant excitation intensities and detector efficiencies. Our instrument has sample holders that can accommodate both liquid and solid (film or powder) samples.

QuantaMaster

Xenon arc lamp steady state excitation
PMT steady state detection
1200 line/mm holographic gratings (excitation blazed at 300 nm; emission blazed at 400 nm)
180-999 nm scan range
0.5 nm resolution
excitation and emission correction

Phosphorescence lifetime upgrade

pulsed Xenon light source
gated PMT detector
50 femptomolar detection limit
400 ns lifetime minimum
0.2-60 points/sec acquisition rate
1-300 Hz excitation pulze frequency
20 ns temporal resolution

Fluorescence lifetime upgrade

LED light sources (280, 310, 340, 375, 395, 405, 435, 460, and 650 nm)
time-domain stroboscopic detection
460 attomolar detection limit
100 ps to 3 µs lifetime range
1.5 ns pulse width
sequential or random acquisition modes

Accessories

Single cuvette Peltier heat/cool stage (-20 to 105 °C)
Front face solid sample holder
Powdered sample holder
Cold finger dewar: liquid nitrogen (77K) and organic solvent slushes; holds NMR tubes
Polarizers (Glan Thompson; 350-600 nm; >77K)
Longpass filters (295, 305, 320, 380, 400, 420, 335, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665, 695, 715, 780, 830, 850, and 1000 nm)