The Leica TCS SP5 microscope utilizes an inverted DMI 6000 microscope which is equipped with a motorized xy stage and epifluorescence illumination. Filter cubes for blue and green excitation (I3, N2.1) are available for visual inspection and focusing of samples. The conventional scanner has one transmitted light detector and three reflected/fluorescence detectors. The excitation laser lines available are 458, 476, 488, 514, 543 and 633 nm and the microscope is equipped with 10x, 40x and 63x dry objectives and a 63x oil immersion objective.
Confocal microscope instructions (updated 8/27/2010)
Key Features:
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inverted DMI 6000 microscope with a motorized xy stage and epifluorescence illumination
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1 transmitted light, 3 reflected/fluorescence PMTs
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excitation laser lines: 458, 476, 488, 514, 543 and 633 nm
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10x, 40x and 63x dry objectives, and 63x oil immersion objective
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high resolution imaging 8k x 8k pixels
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FRAP, FRET measurements
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ROI scan
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spectral un-mixing, deconvolution, colocalization
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3-D visualization
To be trained on this instrument, please contact Dr. Yordanos Bisrat.
CdSe quantum dots (red) coating a protein fiber (green). 3D projection of an image stack taken with the Leica TCS SP5 laser confocal microscope using sequential scanning with excitation wavenlengths 488 nm (EGFP tagged protein fiber emitting at 507 nm) and 458 nm (CdSe emitting at 580 nm) using a 63x objective (dry, N.A. 0.7). Image taken by Ravish Majithia.
