Spectrofluorometer

The PTI QuantaMaster series spectrofluorometer is a modular system with capabilities for measuring many luminescence phenomena.  An arc lamp provides illumination for steady state measurements for observing both emission and excitation scans.  A Xenon arc lamp provides pulsed illumination for measuring phosphorescence lifetimes.  Additionally, several laser and LED sources are available at specific wavelengths that can be attached for collecting fluorescence lifetime measurements.  Felix32 software can be used to collect and analyze excitation and emission scans, excitation and emission ratios, timebased scans for single samples or up to 10 dyes simultaneously, lifetime measurements, and quantum yield.  Furthermore, the Felix32 software can calculate a corrected spectrum to account for non-constant excitation intensities and detector efficiencies.  Our instrument has sample holders that can accommodate both liquid and solid (film or powder) samples.

QuantaMaster

• Xenon arc lamp steady state excitation
• PMT steady state detection
• 1200 line/mm holographic gratings (excitation blazed at 300 nm; emission blazed at 400 nm)
• 180-999 nm scan range
• 0.5 nm resolution
• excitation and emission correction

Phosphorescence upgrade

• pulsed Xenon light source
• gated PMT detector
• 50 femptomolar detection limit
• 400 ns lifetime minimum
• 0.2-60 points/sec acquisition rate
• 1-300 Hz excitation pulze frequency
• 20 ns temporal resolution

Lifetime upgrade

• LED light sources (list wavelengths)
• time-domain stroboscopic detection
• 460 attomolar detection limit
• 100 ps to 3 µs lifetime range
• 1.5 ns pulse width
• sequential or random acquisition modes

Accessories

• Single cuvette Peltier heat/cool stage (-20 to 105 °C)
• Front face solid sample holder
• Powdered sample holder
• Cold finger dewar: liquid nitrogen (77K) and organic solvent slushes; holds NMR tubes
• Polarizers (Glan Thompson; 350-600 nm; >77K)
• Longpass filters (295, 305, 320, 380, 400, 420, 335, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665, 695, 715, 780, 830, 850, and 1000 nm)

QuantaMaster Measurement Capabilities

• Steady state emission/excitation spectra
• Phosporescence decay (lifetime)
• Fluorescence decay (lifetime)
• Gated emission spectra (time-resolved)
• Quantum yield determination (liquid)
• FRET measurements (via lifetime)
• Timebased excitation/emission scanning

Spectrofluorometer Instructions

To be trained on this instrument, please contact Dr. Amanda Young.