Confocal microscopes

Leica TCS SP5

This microscope utilizes an inverted DMI 6000 microscope which is equipped with a motorized xy stage and epifluorescence illumination. Filter cubes for blue and green excitation (I3, N2.1) are available for visual inspection and focusing of samples. The conventional scanner has one transmitted light detector and three reflected/fluorescence detectors. The excitation laser lines available are 458, 476, 488, 514, 543 and 633 nm and the microscope is equipped with 10x, 40x and 63x dry objectives and a 63x oil immersion objective.

Confocal microscope instructions

Leica TCS SP5-RS

The resonant scanner has a scan speed of 8000 Hz (up to 25 frames per second at 512x512 pixels) and enables true confocal imaging and fast frame recording, a prerequisite for quantitative data recording of living samples. The excitation laser lines available are 458, 476, 488, 514, 543 and 633 nm and the microscope is equipped with 10x and 40x dry objectives and a 63x water immersion objective.

Confocal RS microscope instructions

Key Features:

• inverted DMI 6000 microscope with a motorized xy stage and epifluorescence illumination
• 1 transmitted light, 3 reflected/fluorescence PMTs
• excitation laser lines: 458, 476, 488, 514, 543 and 633 nm
• 10x, 40x and 63x dry objectives, 63x oil immersion objective, and a 63x water immrsion objective
• high resolution imaging 8k x 8k pixels
• scans 200 frames per sec
• FRAP, FRET measurements
• ROI scan
• spectral un-mixing, deconvolution, colocalization
• 3-D visualization

To be trained on this instrument, please contact Dr. Amanda Young.